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Image Search Results
Journal: Journal of Cell Communication and Signaling
Article Title: Effects of carbon nanotubes on intercellular communication and involvement of IL-1 genes
doi: 10.1007/s12079-016-0323-0
Figure Lengend Snippet: Immunofluorescence images of Gja1, Gjb1 and Gjb2 in IL1-WT and IL1-KO cells after exposure to CNT-1 and CNT-2. IL1-WT and IL1-KO cells were plated on cover slips and were allowed to attach for 24 h before exposure to CNTs or dispersion media alone for 24 h. Cells were stained with the respective antibodies and fluorescent secondary antibodies were used to detect expression. Hoechst staining was used to visualize cell nuclei. a Representative images for Gja1. b Representative images for Gjb1. c Representative images for Gjb2. Arrows indicate interesting areas with hemichannels and gap junction channels when present between cells. For Gjb1 arrows also point to dividing cells having increased expression of Gjb1. Scale bar: 10 μm
Article Snippet: Antibodies used were as follows; Gja1 (rabbit connexin 43 polyclonal antibody, Cell Signaling Technology),
Techniques: Immunofluorescence, Dispersion, Staining, Expressing
Journal: Journal of Cell Communication and Signaling
Article Title: Effects of carbon nanotubes on intercellular communication and involvement of IL-1 genes
doi: 10.1007/s12079-016-0323-0
Figure Lengend Snippet: Gja1 , Gjb1 and Gjb2 mRNA expression levels in IL1-WT or IL1-KO cells after exposure to dispersion media alone, CNT-1 or CNT-2 . a Gja1 mRNA expression levels investigated by qPCR after exposure to CNT-1 and CNT-2 for 24 h. b Gjb1 mRNA expression levels after exposure to the CNTs for 24 h. c Gjb2 mRNA expression levels after exposure to the CNTs for 24 h. Values represent the mean ± standard error (SE) of three independent experiments performed in triplicate. * P < 0.05 between IL1-WT and IL1-KO cells. # P < 0.05 between exposed IL1-WT or IL1-KO and their respective non-exposed controls
Article Snippet: Antibodies used were as follows; Gja1 (rabbit connexin 43 polyclonal antibody, Cell Signaling Technology),
Techniques: Expressing, Dispersion
Journal: Journal of Cell Communication and Signaling
Article Title: Effects of carbon nanotubes on intercellular communication and involvement of IL-1 genes
doi: 10.1007/s12079-016-0323-0
Figure Lengend Snippet: Localization of Gja1, Gjb1 and Gjb2 protein after CNT exposure. Cytosolic ( c ) and integral membrane/membrane-associated ( m ) proteins were separated into two fractions. Western blot analysis was used to investigate the localization of Gja1, Gjb1 and Gjb2. Antibodies against NaK ATPase α1 and α-Tubulin were used to detect the membrane and cytosolic fractions, respectively. The images shown are representative of two independent experiments
Article Snippet: Antibodies used were as follows; Gja1 (rabbit connexin 43 polyclonal antibody, Cell Signaling Technology),
Techniques: Membrane, Western Blot
Journal: International journal of antimicrobial agents
Article Title: Antibacterial and antivirulence activities of auranofin against Clostridium difficile
doi: 10.1016/j.ijantimicag.2018.09.018
Figure Lengend Snippet: IL-8 level was assessed in Caco-2 cells treated with supernatant containing C. difficile toxins, with or without the addition of auranofin. OD450 coincides with the level of IL-8 in the cell supernatant. Error bars represent standard deviation values from triplicate samples used for each test agent. An asterisk (*) indicates significant difference (P < 0.05) between cells exposed to supernatant containing toxin alone and cells treated with supernatant containing auranofin (1 µg/mL) using one-way (ANOVA) followed by Dunnett’s multiple comparisons test.
Article Snippet:
Techniques: Standard Deviation
Journal: International journal of antimicrobial agents
Article Title: Antibacterial and antivirulence activities of auranofin against Clostridium difficile
doi: 10.1016/j.ijantimicag.2018.09.018
Figure Lengend Snippet: The minimum inhibitory concentration (MIC, µg/mL) of auranofin and control drugs against vancomycin-resistant Enterococcus faecium isolates.
Article Snippet:
Techniques: Concentration Assay
Journal: International journal of antimicrobial agents
Article Title: Antibacterial and antivirulence activities of auranofin against Clostridium difficile
doi: 10.1016/j.ijantimicag.2018.09.018
Figure Lengend Snippet: The minimum inhibitory concentration (MIC, µg/mL) of auranofin and control anticlostridial drugs against C. difficile isolates.
Article Snippet:
Techniques: Concentration Assay